| ECF

Eosinophil chemokines (ECFs) are mainly derived from blood, tissue fluids, and different numbers of cells. The major eosinophil chemokines in the fluid compartment are the complement-derived chitin-free C3a and C5a and, to a lesser extent, the cleavage product of the latter, C5a-desarg.Although both are highly potent chemokines for eosinophils, C5a also activates several other monocyte cell types. Most eosinophil chemokines are derived from resident tissue or peripheral blood cells. They include different chemical entities such as lipids, peptides and proteins.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (pg/mL)	2000	1000	500	250	125	62.5	31.25	0
OD	2.15	1.72	1.4	1	0.67	0.48	0.33	0.1
Corrected OD	2.05	1.62	1.3	0.9	0.57	0.38	0.23	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Rat ECF spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-101	96
Plasma(n=8)	92-105	102
Cell Culture Supernates(n=8)	96-108	105

 

| Linearity

Samples were spiked with high concentrations of Rat ECF and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	84-95	88-96	90-110
	Average (%)	91	93	96
1 : 4	Range (%)	89-103	87-108	105-115
	Average (%)	94	98	108